Particulate organic carbon potentially increases methane emissions from oxic water of eutrophic lakes

Authors

Chuanqiao Zhou, Muchun Zhou, Ruoyu Jia, Yu Peng, Fenjun Zhao, Runze Xu, Shuoyuan Liang, Akihiko Terada, Guoxiang Wang, Tsuyoshi Kinouchi, Xiaoguang Xu

Lakes are hot spots for methane (CH₄) emissions and particulate organic carbon (POC) production, which describes the methane paradox phenomenon. However, the current understanding of the source of POC and its effect on CH₄ emissions during eutrophication remains unclear. In this study, 18 shallow lakes in different trophic states were selected to investigate the POC source and its contribution to CH₄ production, particularly to reveal the underlying mechanisms of the methane paradox. The carbon isotopic analysis showed that the δ¹³C_poc ranged from −30.28 ‰ to −21.14 ‰, indicating that cyanobacteria-derived carbon is an important source of POC. The overlying water was aerobic but contained high concentrations of dissolved CH₄. Particularly, in hyper-eutrophic lakes, such as Lakes Taihu, Chaohu, and Dianshan, the dissolved CH₄ concentrations were 2.11, 1.01, and 2.44 μmol/L, while the dissolved oxygen concentrations were 3.11, 2.92, and 3.17 mg/L, respectively. The intensified eutrophication increased the POC concentration, concomitantly promoting the dissolved CH₄ concentration and the CH₄ flux. These correlations revealed the role of POC in CH₄ production and emission fluxes, particularly as a possible cause of the methane paradox, which is crucial for accurately evaluating the carbon budget and balance in shallow freshwater lakes.

Key factors driving dissolved organic matter composition and bioavailability in lakes situated along the Eastern Route of the South-to-North Water Diversion Project, China

Authors

Yongqiang Zhou, Lili Chen, Lei Zhou, Yunlin Zhang, Kai Peng, Zhijun Gong, Kyoung-Soon Jang, Robert G.M. Spencer, Erik Jeppesen, Justin D. Brookes, Dolly N. Kothawala, Fengchang Wu

The Eastern Route of the South-to-North Water Diversion Project (SNWDP-ER) is a large scale multi-decade infrastructure project aiming to divert substantial amounts of water (≈45 billion m³ yr⁻¹) to alleviate water shortage in comparatively arid regions of northern China. The project has ramifications for hydrological connectivity and biogeochemical cycling of dissolved organic matter (DOM) in regional lakes affected by the project. We carried out an extensive field sampling campaign along the SNWDP-ER in different hydrological seasons of 2018 and monthly observations in Lake Hongze and Lake Luoma from April 2018 to June 2021. We found the lakes connecting to the SNWDP-ER had higher mean DOC, specific UV absorbance, higher ratio of humic-like to protein-like fluorophores (Humic : Protein), and shallower spectral slope (S_275–295) in the wet season compared to the wet-to-dry transition, and dry seasons. The southern lakes and Yangtze River had lower DOC concentration, bioavailable DOC (BDOC), and higher DOM aromaticity compared to the northern two downstream lakes. Ultrahigh-resolution mass spectrometry (FT-ICR MS) revealed higher relative abundance of CHO-containing and aromatic compounds in the Yangtze River and the southern three upstream lakes compared to the northern two lakes. The data from Lake Hongze and Lake Luoma, studied in different hydrological seasons, suggest that water delivery had high consistency in DOM composition and BDOC over the season. We conclude that positioning along the watercourse and seasonally variable hydrological conditions play an important role in influencing the DOM composition and bioavailability of key lakes connecting to the SNWDP-ER. Our results indicated that the water diversion project delivers water with low DOC concentration and higher aromaticity and thus is of higher quality since it has higher DOM removal potential during drinking water treatment.

Patterns in sources and forms of nitrogen in a large eutrophic lake during a cyanobacterial harmful algal bloom

Authors

Jenan J. Kharbush, Rebecca S. Robinson, Susan J. Carter

Western Lake Erie experiences an annual, toxic cyanobacterial harmful algal bloom (cyanoHAB), primarily caused by excess anthropogenic inputs of nitrogen (N) and phosphorous (P). Because the non-N fixing cyanobacteria species Microcystis dominates these blooms, N availability is hypothesized to play a central role in cyanoHAB progression, as well as production of the N-rich toxin microcystin. Many previous studies focused on nitrate because it is the most abundant N substrate during bloom initiation. However, recent work implicated reduced N substrates like ammonium and dissolved organic N (DON) in promoting greater bloom biomass and longevity. To examine the relative importance of oxidized and reduced N substrates to phytoplankton during different bloom stages, we measured concentrations and natural abundance δ¹⁵N isotope values of dissolved N substrates and phytoplankton biomass throughout the entirety of the 2020 cyanoHAB in Western Lake Erie. The results provide the first data on DON dynamics and composition in Western Lake Erie, and suggest that phytoplankton, including Microcystis, likely relied on N regenerated from the DON pool in later bloom stages. In addition, the stable isotope data confirm the importance of nitrate delivered via the Maumee River to cyanobacterial growth and toxin production.

One-step fluorometric determination of multiple-component dissolved organic matter in aquatic environments

Authors

Bo Gong, Wei Chen, Patrick H.-L. Sit, Xian-Wei Liu, Chen Qian

Dissolved organic matter (DOM) is ubiquitous in aqueous environments and is composed of different components that play different but important roles in the migration and the fate of pollutants, emergence of the disinfect byproduct, thus requiring quantitative characterization. However, until now, simultaneous quantification of the main contents in DOM, i.e., saccharides, proteins, and humic substances, has been difficult, impeding us from understanding and predicting the environmental behaviors of typical pollutants. In this work, a fluorescence approach based on the excitation emission matrix (EEM), combined with a new algorithm, denoted matrix reconstruction coupled with prior linear decomposition (MR-PLD), was developed to quantify multiple DOM simultaneously. First, a set of simulated water samples consisting of glucose, tryptones, and humic acid (HA) were analyzed using MR-PLD to validate the feasibility of the method. The DOM components could be reliably determined with a higher accuracy than parallel factor analysis (PARAFAC) and Parallel Factor Framework-Linear Regression (PFFLR), also with a more convenient procedure than conventional PLD. Second, both actual simulated and experimental methods were performed to test the anti-interference performance of MR-PLD, indicating that the quantification of DOM would not be significantly impacted by other fluorophores. Finally, several actual water samples from natural waters and wastewater treatment plants were also analyzed to confirm the robustness of this method in actual aqueous environments. This study provides a new approach to characterize DOM with EEM, contributing to its convenient concentration monitoring and the further exploration of the environmental impacts.

Emission of cyanobacterial volatile organic compounds and their roles in blooms

Emission of cyanobacterial volatile organic compounds and their roles in blooms

Author

Zhaojiang Zuo

Cyanobacteria are photosynthetic prokaryotes and one of dominant species in eutrophicated waters, which easily burst blooms in summer with high irradiance and temperature conditions. In response to high irradiance, high temperature, and nutrient conditions, cyanobacteria release abundant of volatile organic compounds (VOCs) by up-regulating related gene expression and oxidatively degrading β-carotene. These VOCs not only increase offensive odor in waters, but also transfer allelopathic signals to algae and aquatic plants, resulting in cyanobacteria dominating eutrophicated waters. Among these VOCs, β-cyclocitral, α-ionone, β-ionone, limonene, longifolene, and eucalyptol have been identified as the main allelopathic agents, which even directly kill algae by inducing programmed cell death (PCD). The VOCs released from cyanobacteria, especially the ruptured cells, exhibit repelling effects on the herbivores, which is beneficial to survival of the population. Cyanobacterial VOCs might transfer aggregating information among homogeneous species, so the acceptors initiate aggregation to resist the coming stresses. It can be speculated that the adverse conditions can promote VOC emission from cyanobacteria, which play important roles in cyanobacteria dominating eutrophicated waters and even bursting blooms.

Photodegradation of organic micropollutants in aquatic environment: Importance, factors and processes

Authors

Zhongyu Guo, Dilini Kodikara, Luthfia Shofi Albi, Yuta Hatano, Guo Chen, Chihiro Yoshimura, Jieqiong Wang

Photochemical reactions widely occur in the aquatic environment and play fundamental roles in aquatic ecosystems. In particular, solar-induced photodegradation is efficient for many organic micropollutants (OMPs), especially those that cannot undergo hydrolysis or biodegradation, and thus can mitigate chemical pollution. Recent reports indicate that photodegradation may play a more important role than biodegradation in many OMP transformations in the aquatic environment. Photodegradation can be influenced by the water matrix such as pH, inorganic ions, and dissolved organic matter (DOM). The effect of the water matrix such as DOM on photodegradation is complex, and new insights concerning the disparate effects of DOM have recently been reported. In addition, the photodegradation process is also influenced by physical factors such as latitude, water depth, and temporal variations in sunlight as these factors determine the light conditions. However, it remains challenging to gain an overview of the importance of photodegradation in the aquatic environment because the reactions involved are diverse and complex. Therefore, this review provides a concise summary of the importance of photodegradation and the major processes related to the photodegradation of OMPs, with particular attention given to recent progress on the major reactions of DOM. In addition, major knowledge gaps in this field of environmental photochemistry are highlighted.

Properties and metal binding behaviors of sediment dissolved organic matter (SDOM) in lakes with different trophic states along the Yangtze River Basin: A comparison and summary

Authors

Tuantuan Fan, Xin Yao, Zhaoli Sun, Dongling Sang, Li Liu, Huanguang Deng, Yinghao Zhang

The nature of sediment dissolved organic matter (SDOM) can reflect the environmental background, nutritional status and human activities and is an important part of lakes. The differences in the binding capacity of heavy metals and organic matter in lake sediments with different trophic states at the catchment scale and the mechanism of the differences in binding are still unclear. To solve this problem, we collected bulk SDOMs (< 0.7 μm) from 6 respective lakes (from upstream to downstream) in the Yangtze River Basin (YRB) to qualitatively and quantitatively characterize their properties and metal binding behaviors using excitation-emission matrix spectroscopy combined with parallel factor analysis (EEM-FARAFAC) and two-dimensional correlation spectroscopy of synchronous fluorescence spectroscopy and Fourier transform infrared spectroscopy (2D-SF-COS and 2D-FTIR-COS). The results showed that sediment dissolved organic carbon (SDOC) was mainly enriched in low molecular weight (LMW: < 1 kDa) fractions. The total fluorescence intensity (F_max) of SDOM from upstream was larger than that from downstream (p = 0.033), and humic-like fluorophores were dominant in these lakes. The F_max of sediment humic-like components (C1+C2) was closely related to the trophic levels of the lakes. Protein-like substances and oxygen-containing functional groups (C−OH, C=O, and C−O) were preferred in the reaction between SDOM and copper (Cu²⁺) or cadmium (Cd²⁺), while a unique binding path was exhibited in the moderately eutrophic DCL. In terms of fluorophore types, higher Cu²⁺-binding abilities (LogK_Cu) were observed in the humic-like matter for the lakes in the upper reaches and tryptophan-like matter for the lakes from the midstream and downstream areas of the YRB. Although Cd²⁺ complexed only with humic-like matter, LogK_Cd was higher than LogK_Cu. In terms of molecular weight (MW), the LogK_Cu/Cd of components were enhanced after MW fractionation. The HMW (0.7 μm – 1 kDa) components possessed higher LogK_Cu in most lakes (except for CHL and C4). The different fluorophores and molecular weight fractions in SDOM make an important contribution to reducing the ecological risks of heavy metals in lakes.

Increasing air temperature relative to water temperature makes the mixed layer shallower, reducing phytoplankton biomass in a stratified lake

Authors

Salla A. Ahonen, Jukka Seppälä, Juha S. Karjalainen, Jonna Kuha, Anssi V. Vähätalo

1. The depth of the mixed layer is a major determinant of nutrient and light availability for phytoplankton in stratified waterbodies. Ongoing climate change influences surface waters through meteorological forcing, which modifies the physical structure of fresh waters including the mixed layer, but effects on phytoplankton biomass are poorly known.
2. To determine the responses of phytoplankton biomass to the depth of the mixed layer, light availability and associated meteorological forcing, we followed daily changes in weather and water column properties in a boreal lake over the first half of a summer stratification period.
3. Phytoplankton biomass increased with the deepening of the mixed layer associated with high wind speeds and low air temperature relative to the temperature of the mixed layer (T_air−T_mix < 0), whereas heatwave conditions—shallow mixed layer driven by high T_air−T_mix value and low wind speed—reduced the biomass.
4. Improving light availability from low to moderate light conditions increased the phytoplankton biomass, while the highest light availability was associated with low phytoplankton biomass.
5. Our study demonstrates that the climatic impact-drivers wind speed and T_air−T_mix are major drivers of mixed layer depth, which controlled phytoplankton biomass during the early summer stratification period. Our study suggests that increasing air temperature relative to water temperature and declining wind speeds have potential to lead to reduced phytoplankton biomass due to a shallower mixed layer during the first half of the stratification period in non-eutrophic lakes with sufficient light availability.

Response of dissolved organic matter to thermal stratification and environmental indication: The case of Gangnan Reservoir

Authors

Ziwei Zhang, Jiajing Meng, Zhaoying Chen, Shilei Zhou, Tianna Zhang, Zhe Chen, Yilin Liu, Jiansheng Cui

Dissolved Organic Matter (DOM), an important part of the carbon cycle in reservoir ecosystems, has a great impact on aquatic environment to recognize the conversion process of different media DOM. The distribution, spectral characteristics, and sources of DOM in Gangnan Reservoir during thermal stratification were analyzed using ultraviolet-visible absorption spectroscopy and excitation-emission matrix spectroscopy. Three humic-like components (C2, C3, and C4) and two protein-like components (C1 and C5) were identified. The proportions of the humic-like components increased with the progression of thermal stratification (C2 and C3 were dominant), whereas the protein-like components decreased in proportion, and the trend in the interstitial water was constant (C3 and C4 were dominant). The proportion of the humic-like components in the sediments was highest during the stationary period of thermal stratification (C2 and C3 were dominant). C2 and C3 were significantly correlated in the water body and interstitial water (P < 0.001), while C1 and C5 were correlated in the sediment (P < 0.05). In the water body, C2 and C3 were negatively correlated during the formative period of thermal stratification (slope = −1.85; R² = 0.52), strongly positively correlated during the stationary period (slope = 0.76; R² = 0.94), and positively correlated during the weakening period of thermal stratification (slope = 0.46; R² = 0.30). With the progression of thermal stratification, the relative contribution of endogenous substances decreased gradually, whereas the humification degree increased in the water body and interstitial water. The protein-like components and key physicochemical factors (Fe, Mn, TN, TP, and COD_Mn) were significantly correlated during the formative period (P < 0.05), and humic-like components and key physicochemical factors (NO₂⁻-N and TN) were significantly correlated during the stationary and weakening periods (P < 0.05). C1, C4, and C5 indicated NO₃⁻-N during the formative period; C2 and C3 indicated NO₃⁻-N during the stationary period; and C2 and C4 indicated NO₃⁻-N during the weakening period in the water body. These findings enhance the understanding the mutual transformation processes of DOM in reservoir ecosystems and could guide water quality management.

Effects of Hypoxia on Coupled Carbon and Iron Cycling Differ Between Weekly and Multiannual Timescales in Two Freshwater Reservoirs

Authors

Abigail S. L. Lewis, Madeline E. Schreiber, B. R. Niederlehner, Arpita Das, Nicholas W. Hammond, Mary E. Lofton, Heather L. Wander, Cayelan C. Carey

Freshwater lakes and reservoirs play a disproportionate role in the global organic carbon (OC) budget, as active sites for carbon processing and burial. Associations between OC and iron (Fe) are hypothesized to contribute substantially to the stabilization of OC in sediment, but the magnitude of freshwater Fe-OC complexation remains unresolved. Moreover, global declines in bottom-water oxygen concentrations have the potential to alter OC and Fe cycles in multiple ways, and the net effects of low-oxygen (hypoxic) conditions on OC and Fe are poorly characterized. Here, we measured the pool of Fe-bound OC (Fe-OC) in surficial sediments from two eutrophic reservoirs, and we paired whole-ecosystem experiments with sediment incubations to determine the effects of hypoxia on OC and Fe cycling over multiple timescales. Our experiments demonstrated that short periods (2–4 weeks) of hypoxia can increase aqueous Fe and OC concentrations while decreasing OC and Fe-OC in surficial sediment by 30%. However, exposure to seasonal hypoxia over multiple years was associated with a 57% increase in sediment OC and no change in sediment Fe-OC. These results suggest that the large sediment Fe-OC pool (∼30% of sediment OC in both reservoirs) contains both oxygen-sensitive and oxygen-insensitive fractions, and over multiannual timescales OC respiration rates may play a more important role in determining the effect of hypoxia on sediment OC than Fe-OC dissociation. Consequently, we anticipate that global declines in oxygen concentrations will alter OC and Fe cycling, with the direction and magnitude of effects dependent upon the duration of hypoxia.