Mitigating the global expansion of harmful cyanobacterial blooms: Moving targets in a human- and climatically-altered world

Authors

Hans W. Paerl, Malcolm A. Barnard

Cyanobacterial harmful algal blooms (CyanoHABs) are a major threat to human and environmental health. As global proliferation of CyanoHABs continues to increase in prevalence, intensity, and toxicity, it is important to identify and integrate the underlying causes and controls of blooms in order to develop effective short- and long-term mitigation strategies. Clearly, nutrient input reductions should receive high priority. Legacy effects of multi-decadal anthropogenic eutrophication have altered limnetic systems such that there has been a shift from exclusive phosphorus (P) limitation to nitrogen (N) limitation and N and P co-limitation. Additionally, climate change is driving CyanoHAB proliferation through increasing global temperatures and altered precipitation patterns, including more extreme rainfall events and protracted droughts. These scenarios have led to the “perfect storm scenario”; increases in pulsed nutrient loading events, followed by persistent low-flow, long water residence times, favoring bloom formation and proliferation. To meet the CyanoHAB mitigation challenge, we must: (1) Formulate watershed and airshed-specific N and P input reductions on a sliding scale to meet anthropogenic and climatic forcings. (2) Develop CyanoHAB management strategies that incorporate current and anticipated climatic changes and extremes. (3) Make nutrient management strategies compatible with other physical-chemical-biological mitigation approaches, such as altering freshwater flow and flushing, dredging, chemical applications, introduction of selective grazers, etc. (4) Target CyanoHAB toxin production and developing management approaches to reduce toxin production. (5) Develop broadly applicable long-term strategies that incorporate the above recommendations.

Unabated Nitrogen Pollution Favors Growth of Toxic Cyanobacteria over Chlorophytes in Most Hypereutrophic Lakes

Authors

Matthew J. Bogard, Richard J. Vogt, Nicole M. Hayes, Peter R. Leavitt

Human release of reactive nitrogen (N) to the environment has increased 10-fold since 1860 and is expected to increase by a further ∼75% by 2050. Much of this N enters phosphorus (P)-rich, eutrophic lakes in agricultural and urbanized watersheds. While N pollution of eutrophic lakes can promote toxic cyanobacterial growth, some cases of extreme N loading have led to the dominance of chlorophytes (green algae). As N loads required to shift communities from cyanobacterial to chlorophyte dominance are unclear, we experimentally tested phytoplankton responses to a gradient of N loading in a P-rich lake. Low-to-moderate doses (1–3 mg N L^–1 week^–1) promoted toxic cyanobacterial dominance and elevated concentrations of the hepatotoxin microcystin. Conversely, loads characteristic of pure urban or agricultural effluents (up to 18 mg N L^–1 week^–1) led to the dominance of chlorophytes over cyanobacteria and lower microcystin content. This indicates that N loads needed to sustain chlorophyte dominance are uncommon, likely restricted to select shallow lakes directly exposed to urban or agricultural effluents. As most N pollution regimes in P-rich lakes will favor toxic cyanobacterial dominance, restricting future N pollution will help curb further cyanobacterial dominance in lakes both directly and by constraining the capacity for future P loading and climate warming to drive cyanobacterial growth.

Photocatalytic degradation of organic micropollutants: Inhibition mechanisms by different fractions of natural organic matter

Authors

Dion Awfa, Mohamed Ateia, Manabu Fujii, Chihiro Yoshimura

Natural organic matter (NOM) can inhibit the photocatalytic degradation of organic micropollutants (OMPs) through inner filter effect, reactive oxygen species (ROS) scavenging, and competitive adsorption. However, previous studies have focused solely on the bulk properties of NOM and our understanding of the inhibition mechanism by NOM fractions during photocatalytic degradation of OMP is still fragmentary. In this study, five well-characterized different NOM samples (i.e., secondary treated wastewater, river water, and three standard NOM surrogates) were used to elucidate the inhibition mechanisms during photocatalytic degradation of carbamazepine (a model OMP) using TiO₂ and its composites with carbon nanotubes (CNT-TiO₂) under UVC and solar-light irradiation. The results indicated that terrestrially derived NOM with high aromaticity, a low oxygen/carbon atom ratio, and large molecular weight is the major fraction that participates in ROS scavenging, competitive adsorption, and inner filter effect. Furthermore, the modeling analysis suggested that inner filter effect due to NOM and ROS scavenging was the most influential inhibitory mechanism. In the case of secondary treated wastewater, the presence of high concentrations of inorganic species (e.g., PO₄³⁻, Cl⁻, and NO₃⁻) together with NOM significantly reduced the photocatalytic degradation of carbamazepine. Overall, the methods and the results of this study provide a comprehensive understanding of the effects of NOM fractions on photocatalysis and highlight the need to further consider the interplay between NOM and background inorganic constituents in photocatalytic degradation of OMP.

Environmental behavior of methamphetamine and ketamine in aquatic ecosystem: Degradation, bioaccumulation, distribution, and associated shift in toxicity and bacterial community

Authors

Zhenglu Wang, Sheng Han, Min Cai, Peng Du, Zhaobin Zhang, Xiqing Li

Ketamine (KET) and methamphetamine (METH) have been recognized as emerging contaminants in aquatic ecosystems. This paper aimed to investigate the environmental behaviour, including the degradation, distribution, and bioaccumulation, of METH, KET, and their main metabolites (amphetamine (AMP) and norketamine (NorKET)). The changes in acute toxicity in the aqueous phase and in the bacterial community in sediment were determined to assess the associated eco-risk of the drug exposure. Five types of lab-scale aquatic ecosystems were established and exposed to KET or METH for 40 days: a water- sediment- organisms- KET system (K), a water- sediment- organisms- METH system (M), a water- sediment- organism- METH- KET system (M + K), a water-sediment- KET- METH system (control), and a water- sediment- organisms system (biocontrol). The results demonstrated that much faster degradation occurred for both METH (t_1/2 = 3.89 and 2.37 days in the M and M + K group, respectively) and KET(t_1/2 = 5.69 days 5.39 days in the K group and M + K group, respectively) than in the control group (t_1/2 = 7.83 and 86.71days for METH and KET, respectively). Rapid adsorption of KET, METH, and their metabolites was observed in the sediment, which had clay and silt as the main particle sizes. KET was observed to be absorbed by shallow-water fish (Chinese medaka, rosy bitterling and mosquito fish), while METH was dominantly ingested by bottom-dwellers (loach). Duckweed might play a crucial role in the dissipation process of METH and KET, which were mainly adsorbed by duckweed roots. During incubation, the acute toxic levels in the K and M + K groups changed from non-toxic to medium toxicity levels, and the toxicity in the M and control groups changed from non-toxic to low toxicity levels. Moreover, marked changes in the bacterial community in the sediment induced by METH or KET exposure were observed, and the most significant change in the bacterial community was observed in the group spiked with both METH and KET. This work for the first time elucidated the environmental behaviors of METH and KET in aquatic ecosystem and associated the impact on ecological system equilibrium.

Microplastics entering northwestern Lake Ontario are diverse and linked to urban sources

Authors

Jelena Grbić, Paul Helm, Samantha Athey, Chelsea M. Rochman

The sources of microplastics and other anthropogenic particles in freshwater are not well understood. The Greater Toronto Area, Canada’s most populous urban area, offers a great study area for understanding the sources and pathways for microplastics to enter freshwater ecosystems. Here, we quantified and characterized microplastics and other anthropogenic particles from Lake Ontario surface waters and source waters (including stormwater runoff, agricultural runoff, and treated wastewater effluent) to better understand sources to the Great Lakes. Anthropogenic particle concentrations in lake samples were 0.8 particles L⁻¹. In source waters, average concentrations were relatively higher in stormwater and wastewater, with 15.4 particles L⁻¹ and 13.3 particles L⁻¹, respectively, compared to 0.9 particles L⁻¹ on average in agricultural runoff. Source waters revealed distinct signatures related to the morphologies of anthropogenic particles, e.g., fibers in wastewater. In addition, many upstream watershed characteristics were found to be significant predictors of anthropogenic particle concentration. Proximity to urban areas were positively correlated to anthropogenic particle concentrations. Future studies should focus on local source-apportionment to inform management and prevent further contamination of microplastics to freshwater ecosystems.

Nanoplastics Promote Microcystin Synthesis and Release from Cyanobacterial Microcystis aeruginosa

Authors

Li-Juan Feng, Xiao-Dong Sun, Fan-Ping Zhu, Yue Feng, Jian-Lu Duan, Fu Xiao, Xiang-Yu Li, Yi Shi, Qian Wang, Jia-Wen Sun, Xiao-Yu Liu, Jia-Qi Liu, Lin-Lin Zhou, Shu-Guang Wang, Zhaojun Ding, Huiyu Tian, Tamara Susan Galloway, Xian-Zheng Yuan

Although the fate of nanoplastics (<100 nm) in freshwater systems is increasingly well studied, much less is known about its potential threats to cyanobacterial blooms, the ultimate phenomenon of eutrophication occurrence worldwide. Previous studies have evaluated the consequences of nanoplastics increasing the membrane permeability of microbes, however, there is no direct evidence for interactions between nanoplastics and microcystin; intracellular hepatotoxins are produced by some genera of cyanobacteria. Here, we show that the amino-modified polystyrene nanoplastics (PS-NH₂) promote microcystin synthesis and release from Microcystis aeruginosa, a dominant species causing cyanobacterial blooms, even without the change of coloration. We demonstrate that PS-NH₂ inhibits photosystem II efficiency, reduces organic substance synthesis, and induces oxidative stress, enhancing the synthesis of microcystin. Furthermore, PS-NH₂ promotes the extracellular release of microcystin from M. aeruginosa via transporter protein upregulation and impaired cell membrane integrity. Our findings propose that the presence of nanoplastics in freshwater ecosystems might enhance the threat of eutrophication to aquatic ecology and human health.

Regional-scale investigation of dissolved organic matter and lead binding in a large impacted lake with a focus on environmental risk assessment

Authors

Xiaokai Zhang, Boling Li, Jianming Deng, Boqiang Qin, Mona Wells, Boris Tefsen

Environmental risk assessment (ERA) increasingly relies on speciation modeling of bioavailability. Heavy metals are the most prevalent pollutants globally, and dissolved organic matter (DOM) plays an important role in speciation and bioavailability of heavy metals. Due to the variation of DOM properties in natural aquatic systems, improvements to the standard one-size-fits-all approach to modeling metal-DOM interactions are needed for ERA. In this study, we investigate variations in DOM and lead (Pb)-DOM binding in Lake Tai (Taihu), a large, impacted lake in eastern China that is characterized by a complex drainage network and is an important water resource at a regional level, and we assess implications of our findings within the context of ERA needs. In our study, DOM in water samples collected from across the 2,400 km² area of Taihu was characterized using three-dimensional excitation-emission matrix and synchronous fluorescence spectroscopy spectra, the latter being used to calculate conditional stability constants for metal binding. Parallel factor analysis and peak picking were used to assess contributions of protein- and humic-like components of DOM, and fluorescence indices indicative of diagenetic processes were calculated. These quantities calculated from spectroscopic studies, in addition to water quality parameters, were analyzed by bivariate and multivariate analysis. Results show that different DOM components are highly variable across different regions of Taihu, and bivariate and multivariate analyses confirm that water quality and DOM characterization parameters are strongly interrelated. This reflects the different inputs, diagenetic and transport processes across the large expanse of Taihu. We find that the conditional stability constant of Pb-DOM binding is strongly affected by the water chemical properties and composition of DOM, though the conditional stability constant is not itself a parameter that differentiates lake water properties in different regions of the lake. The variability of DOM composition and Pb-DOM binding strength across Taihu is consistent with prior findings that a one-size-fits-all approach to metal-DOM binding may lead to inaccuracies in commonly used speciation models, and therefore such generalized approaches need improvement for regional-level ERA in complex watersheds. The approach taken here to obtain site-specific metal-DOM conditional stability constants for use in increasing the accuracy of speciation modeling is fit-for-purpose for ERA applications at regional levels because the approach is relatively simple, inexpensive, and amenable to high throughput analysis.

 

Total phosphorus export from Iowa agricultural watersheds: Quantifying the scope and scale of a regional condition

Authors

Keith E. Schilling, Matthew T. Streeter, Anthony Seeman, Christopher S. Jones, Calvin F. Wolter

Total phosphorus (TP) export in rivers contributes to development of hypoxic conditions in receiving waterbodies and is typically sourced to agricultural nonpoint sources. Systematic quantification of TP loads and yields at a regional scale is not often reported but TP yields are known to vary considerably. In this study, TP yields from 46 Iowa watersheds were evaluated for the period from 2000 to 2017 to evaluate long-term patterns of TP export and quantify the contribution of Iowa-sourced TP loads to the Gulf of Mexico. Using two common load estimation programs, annual mean and median TP yields ranged from 0.44 to 7.71 kg/ha and 0.32 to 3.75 kg/ha, respectively. Highest median yields were found in western and southern Iowa. The weighted average TP yield for Iowa was 1.70 kg/ha and, on average, Iowa’s TP export contributes approximately 15% of the TP load to the Gulf of Mexico. Reducing TP export will require improved quantification and differentiation of sources of TP loads in Midwestern rivers and a commitment to find strategies that reduce both TP and NO₃-N loss from agricultural lands in a complementary and additive approach.

A new approach to the biological monitoring of freshwater systems: Mapping nutrient loading in two South African rivers, a case study

Authors

Samuel N. Motitsoe, Martin P. Hill, Trevor S. Avery, Jaclyn M. Hill

Excessive addition of nitrogen (N) has threatened aquatic ecosystems for decades. Traditional water quality and biological monitoring assessment tools are widely used for monitoring nutrient loads and ecosystem health, but most of these methods cannot distinguish between different types and sources of pollution. This is a challenge, particularly when dealing with non-point sources of anthropogenic nitrogen inputs into freshwater systems. Recent laboratory studies using stable isotopic ratios (δ¹⁵N and C/N) of aquatic macrophytes (duckweed: Spirodela spp.) have shown successful differentiation and mapping between different N-sources and further, showed abilities to act as early warning indicators for environmental N-loading. Therefore, the aim of this study was to field test the potential of stable isotopic values of transplanted Spirodela spp. to map temporal and spatial N-loading variation and determine the main sources of N-loading in two river systems in the Eastern Cape Province of South Africa, using previously grown, isotopically calibrated and transplanted Spirodela plants, collected over a 13-month sampling period. Nitrogen isotopic values (δ¹⁵N) of Spirodela plants traced environmental N-loading and identified pollution hotspots and sources through time and space over a wide range of nutrient gradients. δ¹⁵N isotopic values of Spirodela spp. provided detailed dynamics on N-loading, therefore supporting its utilisation in the biological monitoring of ecosystem health and the early detection of eutrophication in freshwater systems.

Mitigating eutrophication and toxic cyanobacterial blooms in large lakes: The evolution of a dual nutrient (N and P) reduction paradigm

Authors

Hans W. Paerl, Karl E. Havens, Hai Xu, Guangwei Zhu, Mark J. McCarthy, Silvia E. Newell, J. Thad Scott, Nathan S. Hall, Timothy G. Otten, Boqiang Qin

Cyanobacterial harmful algal blooms (CyanoHABs) are an increasingly common feature of large, eutrophic lakes. Non-N₂-fixing CyanoHABs (e.g., Microcystis) appear to be proliferating relative to N₂-fixing CyanoHABs in systems receiving increasing nutrient loads. This shift reflects increasing external nitrogen (N) inputs, and a > 50-year legacy of excessive phosphorus (P) and N loading. Phosphorus is effectively retained in legacy-impacted systems, while N may be retained or lost to the atmosphere in gaseous forms (e.g., N₂, NH₃, N₂O). Biological control on N inputs versus outputs, or the balance between N₂ fixation versus denitrification, favors the latter, especially in lakes undergoing accelerating eutrophication, although denitrification removal efficiency is inhibited by increasing external N loads. Phytoplankton in eutrophic lakes have become more responsive to N inputs relative to P, despite sustained increases in N loading. From a nutrient management perspective, this suggests a need to change the freshwater nutrient limitation and input reduction paradigms; a shift from an exclusive focus on P limitation to a dual N and P co-limitation and management strategy. The recent proliferation of toxic non-N₂-fixing CyanoHABs, and ever-increasing N and P legacy stores, argues for such a strategy if we are to mitigate eutrophication and CyanoHAB expansion globally.